Quantitating plant microRNA-mediated target repression using a dual-luciferase transient expression system.
نویسندگان
چکیده
microRNA (miRNA) mediated repression of target genes plays essential roles in a variety of functions in plants. An easy-to-use method that can effectively validate functional miRNA-target interactions in plants thus is of particular interest. Here, we describe an Agrobacterium tumefaciens-mediated in vivo assay utilizing a dual-luciferase reporter system. With this method, the strength of miRNA-mediated target repression can be quantified at both the mRNA (via qRT-PCR) and protein (via dual-luciferase assay) levels quickly and accurately.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 1284 شماره
صفحات -
تاریخ انتشار 2015